DICE-TB Xpert Plus panel (Qualitative) by Real Time PCR

DICE-TB Xpert Plus Panel (Qualitative) by Real-Time PCR

DICE-TB Xpert Plus Panel is a molecular diagnostic test designed for the qualitative detection of Mycobacterium tuberculosis (MTB) complex DNA and assessment of rifampicin resistance-associated markers using real-time PCR. Molecular tests such as Xpert-based assays are recommended as important initial diagnostic tools for TB and rifampicin-resistant TB.

What the test detects

• Mycobacterium tuberculosis complex
• Rifampicin resistance-associated markers
• Provides a qualitative molecular result
• Uses Real-Time PCR / nucleic acid amplification technology

Why is it done?

This test may be recommended when tuberculosis is suspected, particularly in patients with symptoms or clinical findings suggestive of pulmonary or extrapulmonary TB. Rapid molecular detection can help clinicians initiate appropriate evaluation and treatment sooner.

Common indications

• Persistent cough
• Fever or night sweats
• Unexplained weight loss
• Blood in sputum
• Suspected pulmonary TB
• Suspected drug-resistant TB
• Clinical suspicion of extrapulmonary TB

Sample

Depending on the clinical indication, specimens may include sputum, BAL, tissue, pus, CSF, synovial fluid or other appropriate specimens. The exact specimen requirement should follow the laboratory's test protocol.

Report interpretation

Results are generally reported qualitatively, such as:

• MTB Detected / Not Detected
• Rifampicin Resistance Detected / Not Detected / Indeterminate, as applicable to the assay

A positive molecular result should be interpreted along with clinical findings and other laboratory investigations.

Key advantage

Real-Time PCR provides rapid molecular detection of TB DNA and can simultaneously provide information relevant to rifampicin resistance, helping guide further diagnostic and therapeutic decisions.

Note: A molecular test result should be interpreted by a qualified physician or microbiologist. Depending on the clinical situation, smear microscopy, culture and additional drug-susceptibility testing may also be required.