Vitamin D - 25-Hydroxy, Serum

Vitamin D - 25-Hydroxy, Serum – V0004c

Test Overview

The Vitamin D - 25-Hydroxy, Serum test is the most accurate and widely recommended blood test to assess your body’s Vitamin D status. It measures the level of 25-hydroxyvitamin D, the primary circulating form of Vitamin D, which reflects both dietary intake and sunlight exposure.

Vitamin D plays a vital role in maintaining bone strength, immune function, and overall metabolic health. Deficiency is very common and can lead to fatigue, bone pain, muscle weakness, and increased risk of infections.

Why This Test is Important

• Helps detect Vitamin D deficiency or insufficiency
• Supports diagnosis of bone-related disorders
• Monitors Vitamin D supplementation therapy
• Assesses calcium metabolism and overall health
• Important for immune system support

Who Should Get This Test

• Individuals with bone pain or muscle weakness
• People with limited sun exposure
• Elderly individuals or postmenopausal women
• Patients with osteoporosis or frequent fractures
• Individuals with chronic illnesses affecting nutrient absorption
• Those on long-term Vitamin D supplements

Sample Type

• Serum (Blood Sample)

Preparation Required

• No fasting required
• Inform your doctor about any medications or supplements

Normal Range (Reference Range)

• Deficiency: Less than 20 ng/mL
• Insufficiency: 20–30 ng/mL
• Sufficient: 30–100 ng/mL
• Toxicity: Above 100 ng/mL

(Note: Reference ranges may vary slightly depending on the laboratory.)

Symptoms of Low Vitamin D

• Fatigue and weakness
• Bone or back pain
• Muscle cramps
• Mood changes or depression
• Frequent infections

Benefits of Testing

• Early detection of deficiency
• Prevents bone disorders like osteoporosis
• Helps optimize Vitamin D supplementation
• Supports better immunity and overall wellness

Reporting Time

• Usually within 24 hours

Test Method

• Chemiluminescent Immunoassay (CLIA) / ECLIA