Dengue NS1 Antigen by ELISA, Serum – D0002e
Test Overview
The Dengue NS1 Antigen test by ELISA is a highly sensitive laboratory test used for the early detection of dengue virus infection. It identifies the presence of the NS1 (Non-Structural Protein 1) antigen, a viral protein released into the bloodstream during the acute phase of infection.
This test is especially valuable in diagnosing dengue within the first few days of fever, even before antibodies are formed.
Why This Test is Done
- Early diagnosis of suspected dengue fever
- Detection during acute febrile phase (Day 1–7)
- Differentiation from other viral infections with similar symptoms
- Monitoring outbreaks and initiating timely treatment
Principle of the Test (ELISA Method)
The test uses the Enzyme-Linked Immunosorbent Assay (ELISA) technique:
- Patient serum is added to wells coated with dengue-specific antibodies
- If NS1 antigen is present, it binds to these antibodies
- A secondary enzyme-linked antibody produces a color reaction
- The intensity of the color is measured to determine positivity
Sample Type
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Specimen: Serum
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Sample Collection: Blood (venipuncture)
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Fasting: Not required
When to Get Tested
- Ideal during first 1–7 days of symptoms
- Symptoms include:
- High fever
- Severe headache
- Joint & muscle pain
- Skin rash
- Nausea or vomiting
NS1 antigen is detectable early and may persist for several days after symptom onset.
Result Interpretation
Positive Result:
- Indicates acute dengue virus infection
- Confirms presence of NS1 antigen in blood
Negative Result:
- Does not completely rule out dengue
- May require follow-up tests (IgM/IgG or PCR) if symptoms persist
Clinical Significance
- Acts as an early marker of dengue infection
- Helps initiate prompt clinical management
- Useful alongside IgM ELISA for improved diagnostic accuracy
Advantages
- Early detection before antibody formation
- High sensitivity during acute phase
- Reliable ELISA-based laboratory method
- Helps reduce complications through timely diagnosis
Turnaround Time
- Typically Same day to 24 hours (lab dependent)
Test Code
D0002e
Precautions
- Early sample collection is crucial
- Negative results may need repeat testing
- Should be interpreted with clinical symptoms
SEO Keywords / Tags
Dengue Test, NS1 Antigen ELISA, Dengue Early Detection, Dengue Blood Test, Viral Fever Test, NS1 Serum Test, Dengue Diagnosis, ELISA Dengue Test, Acute Dengue Screening
