CD 4 Count by Flowcytometry, Bone Marrow

CD4 Count by Flow Cytometry, Bone Marrow

CD4 Count by Flow Cytometry in Bone Marrow is a specialized diagnostic test used to measure the number and percentage of CD4-positive T-lymphocytes present in bone marrow samples. CD4 cells are an important part of the immune system and help coordinate immune responses against infections and diseases.

Purpose of the Test

• Evaluation of immune status
• Assessment of hematological disorders
• Monitoring immune suppression or recovery
• Investigation of lymphoma, leukemia, and bone marrow disorders
• Supportive assessment in HIV/AIDS-related marrow involvement
• Pre- and post-transplant immune monitoring

Principle

The test uses Flow Cytometry, an advanced laser-based technology that identifies and counts CD4-positive cells using fluorescent monoclonal antibodies.

Sample Required

• Bone Marrow Aspirate

Method

• Flow Cytometry

Preparation

• Usually no special preparation required
• Clinical history and medication details may be needed

Reporting Units

• Cells/µL
• Percentage of CD4-positive lymphocytes

Clinical Significance

Abnormal CD4 counts may indicate:

• Immunodeficiency disorders
• HIV-related immune suppression
• Bone marrow infiltration
• Leukemia or lymphoma
• Post-chemotherapy immune suppression
• Autoimmune disorders

Turnaround Time

• Typically 24–72 hours depending on laboratory setup

Recommended For

• Hematology evaluation
• Immunology workup
• Oncology patients
• Bone marrow transplant monitoring